Dr. Élodie STEMPIEN
Attachée Temporaire à l’Enseignement et la Recherche
Courriel : elodie.stempien@uha.fr
Professional Experience
Education
Qualifications and skills
Laboratory Techniques and Scientific Qualification
in vitro in vitro culture:
Grapevine (calli, liquid cells culture), Arabidopsis thaliana, Corn,
and in vivo culture Sunflower. in vivo culture : Arabidopsis thaliana, Corn, wheat (greenhouse)
Cellular biology
Roots microscopic analysis
Mycology
Fungal culture (mycotheca maintenance, inventory management, liquid and solid culture), fungal growth and inhibition tests
Plant Physiology
Germination test, root growth test, morphological analyses
Biochemistry
Precipitation and purification of proteins (dialysis), assay and analysis of secondary metabolites, enzymatic activity tests, HPLC, LC/MS, spectrophotometry and fluorimetry
Molecular Biology
DNA/RNA Extraction, Reverse Transcription, qPCR
Virology
Fungi double-stranded RNA extraction, mycovirus identification and viral curettage
Computer
Microsoft Office, WinRhizo, ImageJ, ImageLab, Bio-Rad CFX Manager, Programming language R for statistical computing, C2i certificate (Internet and computer skills)
Languages
- French : Native language
- English : Fluent
- German : Novice level
- Russian : Novice level
Supervising and mentoring activities
Teaching experience
Teaching fellow for Bachelor’s degree of Agronomy and Food Science and Master’s degree of Valorization and Transformation of Agricultural Productions, Faculty of Marketing and Agrosciences, Colmar, France
- PhD Teaching Mission (11/14 – 11/15)
- Temporary Research and Teaching Assistant (10/16 – 08/17)
- Temporary Research and Teaching Assistant (07/17 – 08/18)
Major courses: Lectures, directed studies and laboratory work on food science, sensory analysis field, biochemistry, plant physiology, genetic engineering, biotechnology (256 hours)
Education mentoring experience
Internship adviser for students preparing their Bachelor’s degree with a major in Agronomy and Food Sciences:
6 students (relationship with internship structures, methodological support, manuscripts corrections and oral presentations)
Research mentoring experience
Mentoring of research trainees from high school level to Master’s degree, 6 research trainees for a total of 30 weeks between 2014 and 2016
Organisation of scientific conferences
Co-organizer of the 5th National Day of Grapevine Trunk Disease, 11/17-18/15, Colmar (France)
In peer-reviewed scientific journals
IN PRESS
M. Bénard-Gellon*, S. Farine*, M.L. Goddard, M. Schmitt, E. Stempien, F. Pensec, H. Laloue, F. Mazet-Kieffer, F. Fontaine, P. Larignon , J. Chong , C. Tarnus and C. Bertsch (2015) Toxicity of extracellular proteins from Diplodia seriata and Neofusicoccum parvum involved in grapevine botryosphaeria dieback. Protoplasma 252:679-687. https://doi.org/10.1007/s00709-014-0716-y. (IF = 2.870)
M. Bénard-Gellon*, M. Ramírez-Suero*, J. Chong, H. Laloue, E. Stempien, E. Abou-Mansour, F. Fontaine, P. Larignon, F. Mazet-Kieffer, S. Farine and C. Bertsch (2014) Extracellular compounds produced by fungi associated with Botryosphaeria dieback induce differential defence gene expression patterns and necrosis in Vitis vinifera cv. Chardonnay cells. Protoplasma 251:1417-1426. https://doi.org/10.1007/s00709-014-0643-y. (IF = 2.870)
Accepted
E. Stempien, M.L. Goddard, Y. Leva, M. Bénard-Gellon, H. Laloue, S. Farine, F. Kieffer-Mazet, C. Tarnus, C. Bertsch and J. Chong (2017) Secreted proteins produced by fungi associated with Botryosphaeria dieback trigger distinct defense responses in Vitis vinifera and Vitis rupestris cells. Protoplasma. doi:10.1007/s00709-017-1175-z. (IF = 2.870)
Submitted
E. Stempien*, M.L. Goddard*, K. Wilhelm, C. Tarnus, C. Bertsch and J. Chong. Grapevine Botryosphaeria dieback fungi have specific aggressiveness factor repertory involved in wood decay and stilbene metabolization.
Submitted to PLoS ONE journal.
* The authors contributed equally to this work.
Invited presentations and conferences
Oral
E. Stempien, M.L. Goddard, Y. Leva, M. Bénard-Gellon, H. Laloue, S. Farine, F. Kieffer-Mazet, C. Bertsch and J. Chong. Defense responses induced by extracellular proteins produced by Botryosphaeriaceae on Vitis cells.
10th International Workshop on Grapevine Trunk Diseases, 07/04-07/17, Reims (France)
M.L. Goddard, E. Stempien, C. Tarnus, C. Bertsch and J. Chong. Impact des protéines extracellulaires de Botryosphaeriaceae sur la production de stilbènes de la vigne.
3rd Phytoday, 06/23/17, Reims (France)
E. Stempien, C. Bertsch and J. Chong. Réactions de défenses induites par les composés extracellulaires des Botryosphaeriaceae sur des cultures cellulaires de Vitis.
Journée Doctorale Science, 07/12/16, Mulhouse (France)
E. Stempien, M. Bénard-Gellon, H. Laloue, S. Farine, F. Mazet-Kieffer, C. Bertsch and J. Chong. Les réactions de défense induites par les composes extracellulaires des Botryosphaeriaceae sur des cultures cellulaires de Vitis. 5th National Day of Grapevine Trunk Disease, 11/17-18/15, Colmar (France)
Poster
E. Stempien, M. Bénard-Gellon, H. Laloue, S. Farine, F. Mazet-Kieffer, C. Bertsch et J. Chong. Effets des composés extracellulaires des Botryosphaeriaceae sur les évènements de signalisation précoces.
Journée Doctorale Science, 07/12/16, Mulhouse (France)
E. Stempien, M. Bénard-Gellon, H. Laloue, S. Farine, F. Mazet-Kieffer, C. Bertsch et J. Chong. Effets des composés extracellulaires des Botryosphaeriaceae sur les évènements de signalisation précoces.
9th Congress of Société Française de Phytopathologie, 06/2-5/15, Colmar (France)
E. Stempien, M. Bénard-Gellon, H. Laloue, S. Farine, F. Mazet-Kieffer, C. Bertsch and J. Chong. Early signaling events induced by Botryosphaeriaceae extracellular compounds.
10th Rhine-Wine Symposium, 05/12-13/15, Karlsruhe (Germany)
E. Stempien, M. Bénard-Gellon, F. Pensec, X. Guan, H. Laloue, S. Farine, F. Mazet-Kieffer, C. Bertsch and J. Chong. Early signaling events induced by grapevine trunk disease fungi.
Plant Biology Day, 10/30/14, Strasbourg (France)
E. Stempien, M. Bénard-Gellon, F. Pensec, X. Guan, H. Laloue, S. Farine, F. Mazet-Kieffer, C. Bertsch and J. Chong. Early signaling events induced by grapevine trunk disease fungi.
9th Rhine-Wine Symposium, 05/15-16/14, Karlsruhe (Germany)
Prizes and awards
Awarded of two grants, by the French society of Phytopathology (SFP) and the Doctoral Program, for the participation and the presentation of an oral presentation at the 10th International Workshop on Grapevine Trunk Disease on July 2013 in Reims, France.
Awarded of the best presentation prize for the oral presentation of my Ph.D. results during the Journée Doctorale Science on the 12th of July 2016 in Mulhouse, France
References available upon request