Dr. Élodie STEMPIEN

Attachée Temporaire à l’Enseignement et la Recherche

Courriel : elodie.stempien@uha.fr

Professional Experience

2017 September - present

University of Haute-Alsace / Faculty of Marketing and Agrosciences / Laboratoire Vigne Biotechnologie et Environnement EA-3991, Colmar, France
Temporary Research and Teaching Assistant
Supervisors: Prof. Christophe Bertsch and Prof. Julie Chong

Understanding the aggression factors of fungi associated with Botryosphaeria dieback: phytoalexin degradation and mycovirus hypothesis

2016 October - 2017 August

University of Haute-Alsace / Faculty of Marketing and Agrosciences / Laboratoire Vigne Biotechnologie et Environnement EA-3991, Colmar, France
Temporary Research and Teaching Assistant
Supervisors: Prof. Christophe Bertsch and Prof. Julie Chong

Study of the interactions between grapevine and the fungi associated with Botryosphaeria dieback: understanding of the induced defense mechanisms and the fungi aggression factors

2013 November - 2017 July

University of Haute-Alsace / Laboratoire Vigne Biotechnologie et Environnement, EA-3991, Colmar, France
Ph.D. Student, Biology of Organisms
Supervisors: Prof. Christophe Bertsch and Prof. Julie Chong

Study of the interactions between grapevine and the fungi associated with Botryosphaeria dieback: understanding of the induced defense mechanisms and the fungi aggression factors

2013 February - 2013 August

INRA-AgroParisTech UMR 1091 Environnement et Grandes Cultures, Thiverval Grignon, France
Graduate Laboratory Internship for Master’s degree
Supervisors: Dr. Marie-Odile Bancal and Dr. Pierre Bancal.

Study of the link between nitrogen remobilization and leaf senescence in order to improve nitrogen function models of wheat cover.

2012 July - 2012 August

Syngenta Crop Protection, Stein, Switzerland
Summer Graduate Researcher
Supervisor: Dr. Raymonde Fonné-Pfister.

Phenotyping of plants and seeds: in vitro culture, germination tests and organogenesis observations, root growth tests, measurement and analysis of morphological roots.

2011 April- 2011 August

Syngenta Crop Protection, Stein, Switzerland
Undergraduate Laboratory Internship for Bachelor’s degree and Summer Graduate Researcher
Supervisor: Dr. Raymonde Fonné-Pfister.

Analysis of the effects of a molecule on the growth and the architecture’s modification of the primary root in Arabidopsis thaliana by in vitro culture and by setting up a method of microscopic analysis of primary root cells, for counting and measuring them.

2010 June - 2010 August

Syngenta Crop Protection, Stein, Switzerland
Summer Undergraduate Researcher
Supervisor: Céline Petit

Purification of chemical synthesis samples for various insecticide and fungicide projects
Use of preparative HPLC Waters auto-purification system
Analysis of crude and pure compounds by LC/MS

2009 July

Syngenta Crop Protection, Stein, Switzerland
Summer Undergraduate Researcher
Supervisor: Céline Petit.

Using Waters HPLC in reverse phase and normal phase
Use and test for efficiency of a freeze-dryer
Analysis of crude and pure compounds by LC/MS

2008 July

Syngenta Crop Protection, Stein, Switzerland
Summer Undergraduate Researcher
Supervisor: Dr. Bettina Böhlendorf.

Using HPLC for synthesis sample separations

Education

2013 November- 2017 July

Ph.D., Biology of Organisms
Thesis: « Étude des interactions entre la vigne et les champignons associés au Botryosphaeria dieback : compréhension des mécanismes de défense induits et facteurs d’agressivité des champignons» (Study of the interactions between grapevine and the fungi associated with Botryosphaeria dieback : understanding of the induced defense mechanisms and the fungi aggression factors), defended on July 13, 2017
Supervisors: Prof. C. Bertsch and Prof. J. Chong
Funded by Alsace Region and Conseil Interprofessionel des Vins d’Alsace
University of Haute-Alsace / Laboratory Vigne Biotechnologies et Environnement EA3991, Colmar

2011 September- 2013 September

Master’s degree of Science with a major in Agronomy and Food Sciences – with honors (2nd out of 22 students)
University of Haute-Alsace / Faculty of Marketing and Agrosciences, Colmar

2007 September- 2011 June

Bachelor’s degree of Science with a major in Agronomy and Food Sciences
University of Haute-Alsace / Faculty of Marketing and Agrosciences, Colmar

Qualifications and skills

Laboratory Techniques and Scientific Qualification

in vitro in vitro culture:
Grapevine (calli, liquid cells culture), Arabidopsis thaliana, Corn,
and in vivo culture Sunflower. in vivo culture : Arabidopsis thaliana, Corn, wheat (greenhouse)

Cellular biology
Roots microscopic analysis

Mycology
Fungal culture (mycotheca maintenance, inventory management, liquid and solid culture), fungal growth and inhibition tests

Plant Physiology
Germination test, root growth test, morphological analyses

Biochemistry
Precipitation and purification of proteins (dialysis), assay and analysis of secondary metabolites, enzymatic activity tests, HPLC, LC/MS, spectrophotometry and fluorimetry

Molecular Biology
DNA/RNA Extraction, Reverse Transcription, qPCR

Virology
Fungi double-stranded RNA extraction, mycovirus identification and viral curettage

Computer
Microsoft Office, WinRhizo, ImageJ, ImageLab, Bio-Rad CFX Manager, Programming language R for statistical computing, C2i certificate (Internet and computer skills)

Languages

  • French : Native language
  • English : Fluent
  • German : Novice level
  • Russian : Novice level

Supervising and mentoring activities

Teaching experience

Teaching fellow for Bachelor’s degree of Agronomy and Food Science and Master’s degree of Valorization and Transformation of Agricultural Productions, Faculty of Marketing and Agrosciences, Colmar, France

  • PhD Teaching Mission (11/14 – 11/15)
  • Temporary Research and Teaching Assistant (10/16 – 08/17)
  • Temporary Research and Teaching Assistant (07/17 – 08/18)

Major courses: Lectures, directed studies and laboratory work on food science, sensory analysis field, biochemistry, plant physiology, genetic engineering, biotechnology (256 hours)

Education mentoring experience

Internship adviser for students preparing their Bachelor’s degree with a major in Agronomy and Food Sciences:
6 students (relationship with internship structures, methodological support, manuscripts corrections and oral presentations)

Research mentoring experience

Mentoring of research trainees from high school level to Master’s degree, 6 research trainees for a total of 30 weeks between 2014 and 2016

Organisation of scientific conferences

Co-organizer of the 5th National Day of Grapevine Trunk Disease, 11/17-18/15, Colmar (France)

In peer-reviewed scientific journalsInvited presentations and conferences

In peer-reviewed scientific journals

IN PRESS

M. Bénard-Gellon*, S. Farine*, M.L. Goddard, M. Schmitt, E. Stempien, F. Pensec, H. Laloue, F. Mazet-Kieffer, F. Fontaine, P. Larignon , J. Chong , C. Tarnus and C. Bertsch (2015) Toxicity of extracellular proteins from Diplodia seriata and Neofusicoccum parvum involved in grapevine botryosphaeria dieback. Protoplasma 252:679-687. https://doi.org/10.1007/s00709-014-0716-y. (IF = 2.870)

M. Bénard-Gellon*, M. Ramírez-Suero*, J. Chong, H. Laloue, E. Stempien, E. Abou-Mansour, F. Fontaine, P. Larignon, F. Mazet-Kieffer, S. Farine and C. Bertsch (2014) Extracellular compounds produced by fungi associated with Botryosphaeria dieback induce differential defence gene expression patterns and necrosis in Vitis vinifera cv. Chardonnay cells. Protoplasma 251:1417-1426. https://doi.org/10.1007/s00709-014-0643-y. (IF = 2.870)

Accepted

E. Stempien, M.L. Goddard, Y. Leva, M. Bénard-Gellon, H. Laloue, S. Farine, F. Kieffer-Mazet, C. Tarnus, C. Bertsch and J. Chong (2017) Secreted proteins produced by fungi associated with Botryosphaeria dieback trigger distinct defense responses in Vitis vinifera and Vitis rupestris cells. Protoplasma. doi:10.1007/s00709-017-1175-z. (IF = 2.870)

Submitted

E. Stempien*, M.L. Goddard*, K. Wilhelm, C. Tarnus, C. Bertsch and J. Chong. Grapevine Botryosphaeria dieback fungi have specific aggressiveness factor repertory involved in wood decay and stilbene metabolization.
Submitted to PLoS ONE journal.

* The authors contributed equally to this work.

Invited presentations and conferences

Oral

E. Stempien, M.L. Goddard, Y. Leva, M. Bénard-Gellon, H. Laloue, S. Farine, F. Kieffer-Mazet, C. Bertsch and J. Chong. Defense responses induced by extracellular proteins produced by Botryosphaeriaceae on Vitis cells.
10th International Workshop on Grapevine Trunk Diseases, 07/04-07/17, Reims (France)

M.L. Goddard, E. Stempien, C. Tarnus, C. Bertsch and J. Chong. Impact des protéines extracellulaires de Botryosphaeriaceae sur la production de stilbènes de la vigne.
3rd Phytoday, 06/23/17, Reims (France)

E. Stempien, C. Bertsch and J. Chong. Réactions de défenses induites par les composés extracellulaires des Botryosphaeriaceae sur des cultures cellulaires de Vitis.
Journée Doctorale Science, 07/12/16, Mulhouse (France)

E. Stempien, M. Bénard-Gellon, H. Laloue, S. Farine, F. Mazet-Kieffer, C. Bertsch and J. Chong. Les réactions de défense induites par les composes extracellulaires des Botryosphaeriaceae sur des cultures cellulaires de Vitis. 5th National Day of Grapevine Trunk Disease, 11/17-18/15, Colmar (France)

Poster

E. Stempien, M. Bénard-Gellon, H. Laloue, S. Farine, F. Mazet-Kieffer, C. Bertsch et J. Chong. Effets des composés extracellulaires des Botryosphaeriaceae sur les évènements de signalisation précoces.
Journée Doctorale Science, 07/12/16, Mulhouse (France)

E. Stempien, M. Bénard-Gellon, H. Laloue, S. Farine, F. Mazet-Kieffer, C. Bertsch et J. Chong. Effets des composés extracellulaires des Botryosphaeriaceae sur les évènements de signalisation précoces.
9th Congress of Société Française de Phytopathologie, 06/2-5/15, Colmar (France)

E. Stempien, M. Bénard-Gellon, H. Laloue, S. Farine, F. Mazet-Kieffer, C. Bertsch and J. Chong. Early signaling events induced by Botryosphaeriaceae extracellular compounds.
10th Rhine-Wine Symposium, 05/12-13/15, Karlsruhe (Germany)

E. Stempien, M. Bénard-Gellon, F. Pensec, X. Guan, H. Laloue, S. Farine, F. Mazet-Kieffer, C. Bertsch and J. Chong. Early signaling events induced by grapevine trunk disease fungi.
Plant Biology Day, 10/30/14, Strasbourg (France)

E. Stempien, M. Bénard-Gellon, F. Pensec, X. Guan, H. Laloue, S. Farine, F. Mazet-Kieffer, C. Bertsch and J. Chong. Early signaling events induced by grapevine trunk disease fungi.
9th Rhine-Wine Symposium, 05/15-16/14, Karlsruhe (Germany)

Prizes and awards

Awarded of two grants, by the French society of Phytopathology (SFP) and the Doctoral Program, for the participation and the presentation of an oral presentation at the 10th International Workshop on Grapevine Trunk Disease on July 2013 in Reims, France.

Awarded of the best presentation prize for the oral presentation of my Ph.D. results during the Journée Doctorale Science on the 12th of July 2016 in Mulhouse, France

 

References available upon request