Dr. Peijie GONG
Post Doctorant au LVBE 2018
Scientific skills / techniques
- Samples collection, isolation of genomic DNA and RNA
- Pathogen cultivation and inoculation
- Suspension cell cultivation and treatment
- TA gene cloning, 5’RACE, 3’RACE, sequence analysis
- RT-PCR, quantitative PCR
- GATEWAY techniques, related vector construction
- Crisp-cas knock out technique
- Transient transformation via biolistic bombantment
- Stable transformation using Agrobacterium-mediated transformation
- Operation of fluorescence confocal microscopy (Spining Disc, Aptom, Zeiss)
- Promoter localization and cloning
- Cis-elements in promoter sequences analysis (PLANTCARE, PLACE)
- Promoter activity assay via dual luciferase reporter system
- Protein extraction and SDS-PAGE analysis
- preparation of tag-fusion protein and polyclonal antibodies
- West-blot analysis
Languages and Software skills
Chinese: Mother tongue
German Basic: B1
Proficient in Windows and Office software (Word, Excel, Powerpoint, Origin, PS, etc.)
Proficient in biology related software: MEGA, Lasergene, Serial Cloner, Primer Premier 5, Bio-Rad CFX manager, Zeiss Microscopy, Leica Microscopy, DNAMAN, and ENDNOTE etc.
Zhang XT*, Gong PJ*(Co-first author), Shi YY, Wang YJ, Zhang CH, 2018. Genetic
Inter-relationships among Chinese Wild Grapes based on SRAP Marker analyses, Vitis, 57, 151–
- Peijie Gong, Yan Li, Yujin Tang, Rong Wei, Zhu Huijun, Yuejin Wang, Chaohong Zhan. Vacuolar processing enzyme (VvβVPE) from Vitis vinifera, processes seed proteins during ovule development, and accelerates seed germination in VvβVPE heterologously over-expressed Arabidopsis. Plant Science, Volume 274, 2018, Pages 420-431, ISSN 0168-9452. https://doi.org/10.1016/j.plantsci.2018.06.023.
- Gong PJ, Riemann M and Nick P 2017. Expression and function analysis of metacaspase gene family during pathogen-triggered PCD in grapevine (being composing)
- Zhang CH*, Gong PJ* (Co-first author), Wei R, Li SX, Zhang XT, Yu YH, Wang YJ. 2013. The metacaspase gene family of Vitis vinifera L. : Characterization and differential expression during ovule abortion in stenospermocarpic seedless grapes. Gene 528, 267-276.
- Tang YJ, Wang RP, Gong PJ, Li SX, Wang YJ, Zhang CH. 2016. Gene Cloning, Expression and Enzyme Activity of Vitis vinifera Vacuolar Processing Enzymes (VvVPEs). Plos One 11.
- Ji W, Li ZQ, Yao WK, Gong PJ, Wang YJ. 2013. Abnormal Seedlings Emerged during Embryo Rescue and Its Remedy for Seedless Grape Breeding. Korean Journal of Horticultural Science & Technology 31, 483-489.
- Gong PJ, Zhang CH, Wei R, Li SX, Wang YJ. 2013. Fusion Expression and Purification of β Type Vacuole Processing Enzyme Gene (VvβVPE) in Vitis vinifera and Its Antibody Preparation. Journal of Agricultural Biotechnology, 21(6): 659-667. (In Chinese)
- Li SX, Zhang CH, Gong PJ, Yang K, Zhang XT, Wang YJ, 2012. Cloning and Expression Analysis of the Differentially Expressed VvWRKY20 Gene in the Development of Seedlessness Grape (Vitis vinifera) Ovule Abortion, Journal of Agricultural Biotechnology, 20(10): 1143-1149 (In Chinese)
- Wei R，Gong PJ，Li SX，Wang YJ，Zhang CH, 2014. Cloning and Expression Analysis of TIAR-like gene in the development of grape (Vitis vinifera) ovule. Journal of Fruit Science, 31（4) : 551-557 (In Chinese)