{"id":893,"date":"2018-04-16T16:01:43","date_gmt":"2018-04-16T14:01:43","guid":{"rendered":"https:\/\/www.lvbe.uha.fr\/\/?page_id=893"},"modified":"2018-06-20T14:15:23","modified_gmt":"2018-06-20T12:15:23","slug":"dr-elodie-stempien","status":"publish","type":"page","link":"https:\/\/www.lvbe.uha.fr\/index.php\/equipe\/membres-de-lequipe\/dr-elodie-stempien\/","title":{"rendered":"Dr. \u00c9lodie STEMPIEN"},"content":{"rendered":"

Attach\u00e9e Temporaire \u00e0 l’Enseignement et la Recherche<\/p>\n

Courriel : elodie.stempien@uha.fr<\/a><\/p>\n

\n
\n

Professional Experience<\/h2>\n
\n
<\/span>2017 September - present<\/div>
\n

University of Haute-Alsace \/ Faculty of Marketing and Agrosciences \/ Laboratoire Vigne Biotechnologie et Environnement EA-3991, Colmar, France
\nTemporary Research and Teaching Assistant
\nSupervisors: Prof. Christophe Bertsch and Prof. Julie Chong<\/p>\n

Understanding the aggression factors of fungi associated with Botryosphaeria dieback: phytoalexin degradation and mycovirus hypothesis<\/p>\n<\/div><\/div>\n

<\/span>2016 October - 2017 August<\/div>
\n

University of Haute-Alsace \/ Faculty of Marketing and Agrosciences \/ Laboratoire Vigne Biotechnologie et Environnement EA-3991, Colmar, France
\nTemporary Research and Teaching Assistant
\nSupervisors: Prof. Christophe Bertsch and Prof. Julie Chong<\/p>\n

Study of the interactions between grapevine and the fungi associated with Botryosphaeria dieback: understanding of the induced defense mechanisms and the fungi aggression factors<\/p>\n<\/div><\/div>\n

<\/span>2013 November - 2017 July<\/div>
\n

University of Haute-Alsace \/ Laboratoire Vigne Biotechnologie et Environnement, EA-3991, Colmar, France
\nPh.D. Student, Biology of Organisms
\nSupervisors: Prof. Christophe Bertsch and Prof. Julie Chong<\/p>\n

Study of the interactions between grapevine and the fungi associated with Botryosphaeria dieback: understanding of the induced defense mechanisms and the fungi aggression factors<\/p>\n<\/div><\/div>\n

<\/span>2013 February - 2013 August<\/div>
\n

INRA-AgroParisTech UMR 1091 Environnement et Grandes Cultures, Thiverval Grignon, France
\nGraduate Laboratory Internship for Master\u2019s degree
\nSupervisors: Dr. Marie-Odile Bancal and Dr. Pierre Bancal.<\/p>\n

Study of the link between nitrogen remobilization and leaf senescence in order to improve nitrogen function models of wheat cover.<\/p>\n<\/div><\/div>\n

<\/span>2012 July - 2012 August<\/div>
\n

Syngenta Crop Protection, Stein, Switzerland
\nSummer Graduate Researcher
\nSupervisor: Dr. Raymonde Fonn\u00e9-Pfister.<\/p>\n

Phenotyping of plants and seeds: in vitro culture, germination tests and organogenesis observations, root growth tests, measurement and analysis of morphological roots.<\/p>\n<\/div><\/div>\n

<\/span>2011 April- 2011 August<\/div>
\n

Syngenta Crop Protection, Stein, Switzerland
\nUndergraduate Laboratory Internship for Bachelor\u2019s degree and Summer Graduate Researcher
\nSupervisor: Dr. Raymonde Fonn\u00e9-Pfister.<\/p>\n

Analysis of the effects of a molecule on the growth and the architecture\u2019s modification of the primary root in Arabidopsis thaliana by in vitro culture and by setting up a method of microscopic analysis of primary root cells, for counting and measuring them.<\/p>\n<\/div><\/div>\n

<\/span>2010 June - 2010 August<\/div>
\n

Syngenta Crop Protection, Stein, Switzerland
\nSummer Undergraduate Researcher
\nSupervisor: C\u00e9line Petit<\/p>\n

Purification of chemical synthesis samples for various insecticide and fungicide projects
\nUse of preparative HPLC Waters auto-purification system
\nAnalysis of crude and pure compounds by LC\/MS<\/p>\n<\/div><\/div>\n

<\/span>2009 July<\/div>
\n

Syngenta Crop Protection, Stein, Switzerland
\nSummer Undergraduate Researcher
\nSupervisor: C\u00e9line Petit.<\/p>\n

Using Waters HPLC in reverse phase and normal phase
\nUse and test for efficiency of a freeze-dryer
\nAnalysis of crude and pure compounds by LC\/MS<\/p>\n<\/div><\/div>\n

<\/span>2008 July<\/div>
\n

Syngenta Crop Protection, Stein, Switzerland
\nSummer Undergraduate Researcher
\nSupervisor: Dr. Bettina B\u00f6hlendorf.<\/p>\n

Using HPLC for synthesis sample separations<\/p>\n<\/div><\/div>\n<\/div>\n<\/div><\/div>\n

\n

Education<\/h2>\n
\n
<\/span>2013 November- 2017 July<\/div>
\n

Ph.D., Biology of Organisms
\nThesis: \u00ab \u00c9tude des interactions entre la vigne et les champignons associ\u00e9s au Botryosphaeria dieback : compr\u00e9hension des m\u00e9canismes de d\u00e9fense induits et facteurs d\u2019agressivit\u00e9 des champignons\u00bb (Study of the interactions between grapevine and the fungi associated with Botryosphaeria dieback : understanding of the induced defense mechanisms and the fungi aggression factors), defended on July 13, 2017
\nSupervisors: Prof. C. Bertsch and Prof. J. Chong
\nFunded by Alsace Region and Conseil Interprofessionel des Vins d\u2019Alsace
\nUniversity of Haute-Alsace \/ Laboratory Vigne Biotechnologies et Environnement EA3991, Colmar<\/p>\n<\/div><\/div>\n

<\/span>2011 September- 2013 September<\/div>
\n

Master\u2019s degree of Science with a major in Agronomy and Food Sciences \u2013 with honors (2nd out of 22 students)
\nUniversity of Haute-Alsace \/ Faculty of Marketing and Agrosciences, Colmar<\/p>\n<\/div><\/div>\n

<\/span>2007 September- 2011 June<\/div>
\n

Bachelor\u2019s degree of Science with a major in Agronomy and Food Sciences
\nUniversity of Haute-Alsace \/ Faculty of Marketing and Agrosciences, Colmar<\/p>\n<\/div><\/div>\n<\/div>\n<\/div><\/div>\n<\/div>\n

\n
\n

Qualifications and skills<\/h2>\n

Laboratory Techniques and Scientific Qualification<\/h3>\n

in vitro in vitro culture:<\/strong>
\nGrapevine (calli, liquid cells culture), Arabidopsis thaliana, Corn,
\nand in vivo culture Sunflower. in vivo culture : Arabidopsis thaliana, Corn, wheat (greenhouse)<\/p>\n

Cellular biology<\/strong>
\nRoots microscopic analysis<\/p>\n

Mycology<\/strong>
\nFungal culture (mycotheca maintenance, inventory management, liquid and solid culture), fungal growth and inhibition tests<\/p>\n

Plant Physiology<\/strong>
\nGermination test, root growth test, morphological analyses<\/p>\n

Biochemistry<\/strong>
\nPrecipitation and purification of proteins (dialysis), assay and analysis of secondary metabolites, enzymatic activity tests, HPLC, LC\/MS, spectrophotometry and fluorimetry<\/p>\n

Molecular Biology<\/strong>
\nDNA\/RNA Extraction, Reverse Transcription, qPCR<\/p>\n

Virology<\/strong>
\nFungi double-stranded RNA extraction, mycovirus identification and viral curettage<\/p>\n

Computer<\/strong>
\nMicrosoft Office, WinRhizo, ImageJ, ImageLab, Bio-Rad CFX Manager, Programming language R for statistical computing, C2i certificate (Internet and computer skills)<\/p>\n

Languages<\/h3>\n
    \n
  • French : Native language<\/li>\n
  • English : Fluent<\/li>\n
  • German : Novice level<\/li>\n
  • Russian : Novice level<\/li>\n<\/ul>\n<\/div><\/div>\n
    \n

    Supervising and mentoring activities<\/h2>\n

    Teaching experience<\/h3>\n

    Teaching fellow for Bachelor\u2019s degree of Agronomy and Food Science and Master\u2019s degree of Valorization and Transformation of Agricultural Productions, Faculty of Marketing and Agrosciences, Colmar, France<\/p>\n

      \n
    • PhD Teaching Mission (11\/14 \u2013 11\/15)<\/li>\n
    • Temporary Research and Teaching Assistant (10\/16 \u2013 08\/17)<\/li>\n
    • Temporary Research and Teaching Assistant (07\/17 \u2013 08\/18)<\/li>\n<\/ul>\n

      Major courses: Lectures, directed studies and laboratory work on food science, sensory analysis field, biochemistry, plant physiology, genetic engineering, biotechnology (256 hours)<\/p>\n

      Education mentoring experience<\/h3>\n

      Internship adviser for students preparing their Bachelor\u2019s degree with a major in Agronomy and Food Sciences:
      \n6 students (relationship with internship structures, methodological support, manuscripts corrections and oral presentations)<\/p>\n

      Research mentoring experience<\/h3>\n

      Mentoring of research trainees from high school level to Master\u2019s degree, 6 research trainees for a total of 30 weeks between 2014 and 2016<\/p>\n

      Organisation of scientific conferences<\/h3>\n

      Co-organizer of the 5th National Day of Grapevine Trunk Disease, 11\/17-18\/15, Colmar (France)<\/p>\n<\/div><\/div>\n<\/div>\n

      In peer-reviewed scientific journals<\/span>Invited presentations and conferences<\/span><\/div>
      \n

      In peer-reviewed scientific journals<\/h2>\n

      IN PRESS<\/h3>\n

      M. B\u00e9nard-Gellon*, S. Farine*, M.L. Goddard, M. Schmitt, E. Stempien, F. Pensec, H. Laloue, F. Mazet-Kieffer, F. Fontaine, P. Larignon , J. Chong , C. Tarnus and C. Bertsch (2015) Toxicity of extracellular proteins from Diplodia seriata and Neofusicoccum parvum involved in grapevine botryosphaeria dieback. Protoplasma 252:679-687. https:\/\/doi.org\/10.1007\/s00709-014-0716-y. (IF = 2.870)<\/p>\n

      M. B\u00e9nard-Gellon*, M. Ram\u00edrez-Suero*, J. Chong, H. Laloue, E. Stempien, E. Abou-Mansour, F. Fontaine, P. Larignon, F. Mazet-Kieffer, S. Farine and C. Bertsch (2014) Extracellular compounds produced by fungi associated with Botryosphaeria dieback induce differential defence gene expression patterns and necrosis in Vitis vinifera cv. Chardonnay cells. Protoplasma 251:1417-1426. https:\/\/doi.org\/10.1007\/s00709-014-0643-y. (IF = 2.870)<\/p>\n

      Accepted<\/h3>\n

      E. Stempien, M.L. Goddard, Y. Leva, M. B\u00e9nard-Gellon, H. Laloue, S. Farine, F. Kieffer-Mazet, C. Tarnus, C. Bertsch and J. Chong (2017) Secreted proteins produced by fungi associated with Botryosphaeria dieback trigger distinct defense responses in Vitis vinifera and Vitis rupestris cells. Protoplasma. doi:10.1007\/s00709-017-1175-z. (IF = 2.870)<\/p>\n

      Submitted<\/h3>\n

      E. Stempien*, M.L. Goddard*, K. Wilhelm, C. Tarnus, C. Bertsch and J. Chong. Grapevine Botryosphaeria dieback fungi have specific aggressiveness factor repertory involved in wood decay and stilbene metabolization.
      \nSubmitted to PLoS ONE journal.<\/p>\n

      * The authors contributed equally to this work.<\/p>\n<\/div>\n

      \n

      Invited presentations and conferences<\/h2>\n

      Oral<\/h3>\n

      E. Stempien, M.L. Goddard, Y. Leva, M. B\u00e9nard-Gellon, H. Laloue, S. Farine, F. Kieffer-Mazet, C. Bertsch and J. Chong. Defense responses induced by extracellular proteins produced by Botryosphaeriaceae on Vitis cells.
      \n10th International Workshop on Grapevine Trunk Diseases, 07\/04-07\/17, Reims (France)<\/p>\n

      M.L. Goddard, E. Stempien, C. Tarnus, C. Bertsch and J. Chong. Impact des prot\u00e9ines extracellulaires de Botryosphaeriaceae sur la production de stilb\u00e8nes de la vigne.
      \n3rd Phytoday, 06\/23\/17, Reims (France)<\/p>\n

      E. Stempien, C. Bertsch and J. Chong. R\u00e9actions de d\u00e9fenses induites par les compos\u00e9s extracellulaires des Botryosphaeriaceae sur des cultures cellulaires de Vitis.
      \nJourn\u00e9e Doctorale Science, 07\/12\/16, Mulhouse (France)<\/p>\n

      E. Stempien, M. B\u00e9nard-Gellon, H. Laloue, S. Farine, F. Mazet-Kieffer, C. Bertsch and J. Chong. Les r\u00e9actions de d\u00e9fense induites par les composes extracellulaires des Botryosphaeriaceae sur des cultures cellulaires de Vitis. 5th National Day of Grapevine Trunk Disease, 11\/17-18\/15, Colmar (France)<\/p>\n

      Poster<\/h3>\n

      E. Stempien, M. B\u00e9nard-Gellon, H. Laloue, S. Farine, F. Mazet-Kieffer, C. Bertsch et J. Chong. Effets des compos\u00e9s extracellulaires des Botryosphaeriaceae sur les \u00e9v\u00e8nements de signalisation pr\u00e9coces.
      \nJourn\u00e9e Doctorale Science, 07\/12\/16, Mulhouse (France)<\/p>\n

      E. Stempien, M. B\u00e9nard-Gellon, H. Laloue, S. Farine, F. Mazet-Kieffer, C. Bertsch et J. Chong. Effets des compos\u00e9s extracellulaires des Botryosphaeriaceae sur les \u00e9v\u00e8nements de signalisation pr\u00e9coces.
      \n9th Congress of Soci\u00e9t\u00e9 Fran\u00e7aise de Phytopathologie, 06\/2-5\/15, Colmar (France)<\/p>\n

      E. Stempien, M. B\u00e9nard-Gellon, H. Laloue, S. Farine, F. Mazet-Kieffer, C. Bertsch and J. Chong. Early signaling events induced by Botryosphaeriaceae extracellular compounds.
      \n10th Rhine-Wine Symposium, 05\/12-13\/15, Karlsruhe (Germany)<\/p>\n

      E. Stempien, M. B\u00e9nard-Gellon, F. Pensec, X. Guan, H. Laloue, S. Farine, F. Mazet-Kieffer, C. Bertsch and J. Chong. Early signaling events induced by grapevine trunk disease fungi.
      \nPlant Biology Day, 10\/30\/14, Strasbourg (France)<\/p>\n

      E. Stempien, M. B\u00e9nard-Gellon, F. Pensec, X. Guan, H. Laloue, S. Farine, F. Mazet-Kieffer, C. Bertsch and J. Chong. Early signaling events induced by grapevine trunk disease fungi.
      \n9th Rhine-Wine Symposium, 05\/15-16\/14, Karlsruhe (Germany)<\/p>\n<\/div><\/div><\/div>\n

      Prizes and awards<\/h2>\n

      Awarded of two grants, by the French society of Phytopathology (SFP) and the Doctoral Program, for the participation and the presentation of an oral presentation at the 10th International Workshop on Grapevine Trunk Disease on July 2013 in Reims, France.<\/p>\n

      Awarded of the best presentation prize for the oral presentation of my Ph.D. results during the Journ\u00e9e Doctorale Science on the 12th of July 2016 in Mulhouse, France<\/p>\n

       <\/p>\n

      References available upon request<\/p>\n","protected":false},"excerpt":{"rendered":"

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