{"id":1564,"date":"2018-05-31T11:25:01","date_gmt":"2018-05-31T09:25:01","guid":{"rendered":"https:\/\/www.lvbe.uha.fr\/\/?page_id=1564"},"modified":"2020-12-02T18:59:52","modified_gmt":"2020-12-02T17:59:52","slug":"dr-peijie-gong","status":"publish","type":"page","link":"https:\/\/www.lvbe.uha.fr\/index.php\/equipe\/chercheurs-venus-collaborer-au-lvbe\/dr-peijie-gong\/","title":{"rendered":"Dr. Peijie GONG"},"content":{"rendered":"<p>Post Doctorant au LVBE 2018<\/p>\n<div class=\"su-row\">\n<div class=\"su-column su-column-size-1-2\"><div class=\"su-column-inner su-u-clearfix su-u-trim\">\n<h2>Education<\/h2>\n<div class=\"su-accordion su-u-trim\">\n<div class=\"su-spoiler su-spoiler-style-default su-spoiler-icon-plus su-spoiler-closed\" data-scroll-offset=\"0\" data-anchor-in-url=\"no\"><div class=\"su-spoiler-title\" tabindex=\"0\" role=\"button\"><span class=\"su-spoiler-icon\"><\/span>Oct.2013-Jun.2017<\/div><div class=\"su-spoiler-content su-u-clearfix su-u-trim\">\n<p><strong>Doctor\u2019s degree in Biology<\/strong><\/p>\n<p>Botanical Institute, Karlsruhe Institute of Technology (KIT), Germany<br \/>\nMajor: Molecular and cell biology<br \/>\nThesis title: Die to Survive, Functional Analysis of Grapevine Metacaspases responsive to ETI (effector-triggered immunity) -related cell death<br \/>\n(Supervisor: Prof. Dr. Peter Nick)<br \/>\nOverall mark: very good (magna cum laude)<\/p>\n<\/div><\/div>\n<div class=\"su-spoiler su-spoiler-style-default su-spoiler-icon-plus su-spoiler-closed\" data-scroll-offset=\"0\" data-anchor-in-url=\"no\"><div class=\"su-spoiler-title\" tabindex=\"0\" role=\"button\"><span class=\"su-spoiler-icon\"><\/span>Sep.2010-Jun.2013<\/div><div class=\"su-spoiler-content su-u-clearfix su-u-trim\">\n<p><strong>Master\u2019s degree in Agronomy<\/strong><\/p>\n<p>College of Horticulture, Northwest A&amp;F University<br \/>\nMajor: Pomology<br \/>\nThesis title: Characterization of Caspase-like Gene Family Related to Ovule Abortion in Grape (<em>Vitis vinifera<\/em>)<br \/>\n(Supervisor: Prof. Zhang Chaohong, Prof. Wang Yuejin)<br \/>\nGPA: 88.3\/100<\/p>\n<\/div><\/div>\n<div class=\"su-spoiler su-spoiler-style-default su-spoiler-icon-plus su-spoiler-closed\" data-scroll-offset=\"0\" data-anchor-in-url=\"no\"><div class=\"su-spoiler-title\" tabindex=\"0\" role=\"button\"><span class=\"su-spoiler-icon\"><\/span>Sep.2006-Jul.2010<\/div><div class=\"su-spoiler-content su-u-clearfix su-u-trim\">\n<p><strong>Bachelor\u2019s degree in Agronomy<\/strong><\/p>\n<p>College of Agronomy, Gansu Agricultural University<br \/>\nMajor: Horticulture<br \/>\nThesis title: The Identification of Genetic Relationship in <a href=\"http:\/\/dict.youdao.com\/search?q=purple&amp;keyfrom=E2Ctranslation\">Purple<\/a> Globe Grape Using RAPD Molecular Marker<br \/>\nGPA: 80.28\/100<\/p>\n<\/div><\/div>\n<\/div>\n<h2>Scientific skills \/ techniques<\/h2>\n<ul>\n<li>Samples collection, isolation of genomic DNA and RNA<\/li>\n<li>Pathogen cultivation and inoculation<\/li>\n<li>Suspension cell cultivation and treatment<\/li>\n<li>TA gene cloning, 5\u2019RACE, 3\u2019RACE, sequence analysis<\/li>\n<li>RT-PCR, quantitative PCR<\/li>\n<li>GATEWAY techniques, related vector construction<\/li>\n<li>Crisp-cas knock out technique<\/li>\n<li>Transient transformation via biolistic bombantment<\/li>\n<li>Stable transformation using Agrobacterium-mediated transformation<\/li>\n<li>Operation of fluorescence confocal microscopy (Spining Disc, Aptom, Zeiss)<\/li>\n<li>Promoter localization and cloning<\/li>\n<li>Cis-elements in promoter sequences analysis (PLANTCARE, PLACE)<\/li>\n<li>Promoter activity assay via dual luciferase reporter system<\/li>\n<li>Protein extraction and SDS-PAGE analysis<\/li>\n<li>preparation of tag-fusion protein and polyclonal antibodies<\/li>\n<li>West-blot analysis<\/li>\n<\/ul>\n<\/div><\/div>\n<div class=\"su-column su-column-size-1-2\"><div class=\"su-column-inner su-u-clearfix su-u-trim\">\n<h2>Research Experience<\/h2>\n<div class=\"su-accordion su-u-trim\">\n<div class=\"su-spoiler su-spoiler-style-default su-spoiler-icon-plus su-spoiler-closed\" data-scroll-offset=\"0\" data-anchor-in-url=\"no\"><div class=\"su-spoiler-title\" tabindex=\"0\" role=\"button\"><span class=\"su-spoiler-icon\"><\/span>Dec.2017 - Present<\/div><div class=\"su-spoiler-content su-u-clearfix su-u-trim\">\n<p><strong>Postdoc position, in Universit\u00e9 de Haute-Alsace (UHA)<\/strong>\uff08supported by Vitifutur project of EU<\/p>\n<p><strong><em>Analysis and engineering of programmed cell death in grapevine trunk disease (GTD)<\/em><\/strong><br \/>\nResponsibilities:<\/p>\n<ul>\n<li>Resolving core question: How to use caspase-like enzyme to rescue our grape host from GTD pathogens infection?<\/li>\n<\/ul>\n<\/div><\/div>\n<div class=\"su-spoiler su-spoiler-style-default su-spoiler-icon-plus su-spoiler-closed\" data-scroll-offset=\"0\" data-anchor-in-url=\"no\"><div class=\"su-spoiler-title\" tabindex=\"0\" role=\"button\"><span class=\"su-spoiler-icon\"><\/span>Oct. 2013 \u2013 Sep. 2017<\/div><div class=\"su-spoiler-content su-u-clearfix su-u-trim\">\n<p><strong>Doctor\u2019s degree dissertation, (supported by Chinese Scholarship Council)<\/strong><\/p>\n<p><strong>Function analysis of <em>metacaspase<\/em> family genes and their promoters during ETI-triggered PCD in <em>Vitis <\/em>defense system<\/strong><br \/>\nResponsibilities:<\/p>\n<ul>\n<li>Setting up expression pattern of each <em>metacaspase<\/em> family member during HR process in grapevine<\/li>\n<li>HR-like necrotic spots observed on <em>Rpv3+<\/em> grapevine induced by <em>viticola <\/em>1191-B15,1137-C20<\/li>\n<li>Clarifying cellular location and cellular function of <em>VrMC2 <\/em>and <em>VrMC5<\/em> in PCD process in <em>rupestris<\/em><\/li>\n<li>Supervising the thesis of bachelor students, master students<\/li>\n<li>Giving exercises and lectures to bachelor students<\/li>\n<\/ul>\n<\/div><\/div>\n<div class=\"su-spoiler su-spoiler-style-default su-spoiler-icon-plus su-spoiler-closed\" data-scroll-offset=\"0\" data-anchor-in-url=\"no\"><div class=\"su-spoiler-title\" tabindex=\"0\" role=\"button\"><span class=\"su-spoiler-icon\"><\/span>Sep. 2011 \u2013 Jun. 2013<\/div><div class=\"su-spoiler-content su-u-clearfix su-u-trim\">\n<p><strong>Part for Master\u2019s degree thesis, (supported by China Agriculture Research System (30)<\/strong><\/p>\n<p>Characterization of metacaspase gene family of <strong><em>vitis vinifera<\/em><\/strong> and their differential expression analysis during ovule abortion process of stenospermocarpic seedlessness<br \/>\nResponsibilities:<\/p>\n<ul>\n<li>Identification and isolation of family genes<\/li>\n<li>Chromosome localization of each gene<\/li>\n<li>Multiple-sequence alignments and phylogenetic analysis<\/li>\n<li>Cis-elements in promoter sequences analysis<\/li>\n<li>Gene expression of metacaspase analyzed by QRT-PCR in different stages of ovule development<\/li>\n<\/ul>\n<\/div><\/div>\n<div class=\"su-spoiler su-spoiler-style-default su-spoiler-icon-plus su-spoiler-closed\" data-scroll-offset=\"0\" data-anchor-in-url=\"no\"><div class=\"su-spoiler-title\" tabindex=\"0\" role=\"button\"><span class=\"su-spoiler-icon\"><\/span>Sep. 2011\u2013 Jun. 2013<\/div><div class=\"su-spoiler-content su-u-clearfix su-u-trim\">\n<p><strong>Part of Master\u2019s degree thesis, (supported by Natural Science Foundation of China (Grant no. 31171926)<\/strong><\/p>\n<p><strong><em>VPE<\/em><\/strong>, a novel <strong>vacuolar processing enzyme<\/strong> gene family from <strong><em>Vitis vinifera<\/em><\/strong>, functions analysis in ovule development process of grapevine<br \/>\nResponsibilities:<\/p>\n<ul>\n<li>The analysis of <em>\u03b2VPE<\/em> gene polymorphism from grapes and the its function with the seedlessness character (in Arabidopsis)<\/li>\n<li>Cloning and analysis of VPE family and the differentially expressed gene in ovule abortion of seedless grape<\/li>\n<li>Function deletion analysis of <em>\u03b2 VPE<\/em> promoter from seedless grapes (in Arabidopsis)<\/li>\n<\/ul>\n<\/div><\/div>\n<\/div>\n<h2>Languages and Software skills<\/h2>\n<p>Chinese: Mother tongue<br \/>\nEnglish: Fluent<br \/>\nGerman Basic: B1<\/p>\n<p>Proficient in Windows and Office software <strong>(Word, Excel, Powerpoint, Origin, PS, etc.)<\/strong><\/p>\n<p>Proficient in biology related software: <strong>MEGA, Lasergene, Serial Cloner, Primer Premier 5, Bio-Rad CFX manager, Zeiss Microscopy, Leica Microscopy, DNAMAN, and ENDNOTE<\/strong> etc.<\/p>\n<\/div><\/div>\n<\/div>\n<h2>Publications<\/h2>\n<ul>\n<li>\n<div>\n<div>\n<div><span class=\"fontstyle0\">Zhang XT*, <\/span><strong><span class=\"fontstyle2\">Gong PJ*(Co-first author)<\/span><\/strong><span class=\"fontstyle0\">, Shi YY, Wang YJ, Zhang CH, <\/span><span class=\"fontstyle3\">2018<\/span><span class=\"fontstyle0\">. Genetic<br \/>\nInter-relationships among Chinese Wild Grapes based on SRAP Marker analyses, <\/span><span class=\"fontstyle4\">Vitis<\/span><span class=\"fontstyle0\">, 57, 151\u2013<br \/>\n157.<\/span><\/div>\n<\/div>\n<\/div>\n<\/li>\n<li><b>Peijie Gong<\/b>, Yan Li, Yujin Tang, Rong Wei, Zhu Huijun, Yuejin Wang, Chaohong Zhan.\u00a0Vacuolar processing enzyme (Vv\u03b2VPE) from Vitis vinifera, processes seed proteins during ovule development, and accelerates seed germination in Vv\u03b2VPE heterologously over-expressed Arabidopsis. Plant Science,\u00a0Volume 274,\u00a02018,\u00a0Pages 420-431,\u00a0ISSN 0168-9452.\u00a0https:\/\/doi.org\/10.1016\/j.plantsci.2018.06.023.<\/li>\n<li><strong>Gong PJ<\/strong>, Riemann M and Nick P 2017. Expression and function analysis of <em>metacaspase<\/em> gene family during pathogen-triggered PCD in grapevine (being composing)<\/li>\n<li>Zhang CH*, <strong>Gong PJ* (Co-first author)<\/strong>, Wei R, Li SX, Zhang XT, Yu YH, Wang YJ. 2013. The metacaspase gene family of Vitis vinifera L. : Characterization and differential expression during ovule abortion in stenospermocarpic seedless grapes. <em>Gene<\/em> 528, 267-276.<\/li>\n<li>Tang YJ, Wang RP, <strong>Gong PJ<\/strong>, Li SX, Wang YJ, Zhang CH. 2016. Gene Cloning, Expression and Enzyme Activity of Vitis vinifera Vacuolar Processing Enzymes (<em>VvVPEs<\/em>). <em>Plos One<\/em> 11.<\/li>\n<li>Ji W, Li ZQ, Yao WK, <strong>Gong PJ<\/strong>, Wang YJ. 2013. Abnormal Seedlings Emerged during Embryo Rescue and Its Remedy for Seedless Grape Breeding. <em>Korean Journal of Horticultural Science &amp; Technology<\/em> 31, 483-489.<\/li>\n<li><strong>Gong PJ<\/strong>, Zhang CH, Wei R,\u00a0Li SX, Wang YJ. 2013. Fusion Expression and Purification of \u03b2 Type Vacuole Processing Enzyme Gene (Vv\u03b2VPE) in <em>Vitis vinifera<\/em> and Its Antibody Preparation. <em>Journal of Agricultural Biotechnology<\/em>, 21(6): 659-667. (In Chinese)<\/li>\n<li>Li SX, Zhang CH, Gong <strong>PJ,<\/strong> Yang K, Zhang XT, Wang YJ, 2012. Cloning and Expression Analysis of the Differentially Expressed <em>VvWRKY20<\/em> Gene in the Development of Seedlessness Grape (<em>Vitis vinifera<\/em>) Ovule Abortion, <em>Journal of Agricultural Biotechnology, <\/em>20(10): 1143-1149 (In Chinese)<\/li>\n<li>Wei R\uff0c<strong>Gong PJ<\/strong>\uff0cLi SX\uff0cWang YJ\uff0cZhang CH, 2014. Cloning and Expression Analysis of TIAR-like gene in the development of grape (<em>Vitis vinifera<\/em>) ovule. <em>Journal of Fruit Science, <\/em>31\uff084) : 551-557 (In Chinese)<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Post Doctorant au LVBE 2018 Publications Zhang XT*, Gong PJ*(Co-first author), Shi YY, Wang YJ, Zhang CH, 2018. Genetic Inter-relationships among Chinese Wild Grapes based on SRAP Marker analyses, Vitis, 57, 151\u2013 157. Peijie Gong, Yan Li, Yujin Tang, Rong Wei, Zhu Huijun, Yuejin Wang, Chaohong Zhan.\u00a0Vacuolar processing enzyme (Vv\u03b2VPE) from Vitis vinifera, processes seed [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":2537,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-1564","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.4 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Dr. Peijie GONG - LVBE - Laboratoire Vigne, Biotechnologies, Environnement | Universit\u00e9 de Haute-Alsace<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.lvbe.uha.fr\/index.php\/equipe\/chercheurs-venus-collaborer-au-lvbe\/dr-peijie-gong\/\" \/>\n<meta property=\"og:locale\" content=\"fr_FR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Dr. Peijie GONG - LVBE - Laboratoire Vigne, Biotechnologies, Environnement | Universit\u00e9 de Haute-Alsace\" \/>\n<meta property=\"og:description\" content=\"Post Doctorant au LVBE 2018 Publications Zhang XT*, Gong PJ*(Co-first author), Shi YY, Wang YJ, Zhang CH, 2018. 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Peijie Gong, Yan Li, Yujin Tang, Rong Wei, Zhu Huijun, Yuejin Wang, Chaohong Zhan.\u00a0Vacuolar processing enzyme (Vv\u03b2VPE) from Vitis vinifera, processes seed [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.lvbe.uha.fr\/index.php\/equipe\/chercheurs-venus-collaborer-au-lvbe\/dr-peijie-gong\/\" \/>\n<meta property=\"og:site_name\" content=\"LVBE - Laboratoire Vigne, Biotechnologies, Environnement | Universit\u00e9 de Haute-Alsace\" \/>\n<meta property=\"article:modified_time\" content=\"2020-12-02T17:59:52+00:00\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Dur\u00e9e de lecture estim\u00e9e\" \/>\n\t<meta name=\"twitter:data1\" content=\"5 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/www.lvbe.uha.fr\\\/index.php\\\/equipe\\\/chercheurs-venus-collaborer-au-lvbe\\\/dr-peijie-gong\\\/\",\"url\":\"https:\\\/\\\/www.lvbe.uha.fr\\\/index.php\\\/equipe\\\/chercheurs-venus-collaborer-au-lvbe\\\/dr-peijie-gong\\\/\",\"name\":\"Dr. Peijie GONG - LVBE - Laboratoire Vigne, Biotechnologies, Environnement | Universit\u00e9 de Haute-Alsace\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/www.lvbe.uha.fr\\\/#website\"},\"datePublished\":\"2018-05-31T09:25:01+00:00\",\"dateModified\":\"2020-12-02T17:59:52+00:00\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/www.lvbe.uha.fr\\\/index.php\\\/equipe\\\/chercheurs-venus-collaborer-au-lvbe\\\/dr-peijie-gong\\\/#breadcrumb\"},\"inLanguage\":\"fr-FR\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/www.lvbe.uha.fr\\\/index.php\\\/equipe\\\/chercheurs-venus-collaborer-au-lvbe\\\/dr-peijie-gong\\\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/www.lvbe.uha.fr\\\/index.php\\\/equipe\\\/chercheurs-venus-collaborer-au-lvbe\\\/dr-peijie-gong\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Accueil\",\"item\":\"https:\\\/\\\/www.lvbe.uha.fr\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"\u00c9quipe\",\"item\":\"https:\\\/\\\/www.lvbe.uha.fr\\\/index.php\\\/equipe\\\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"Chercheurs venus collaborer au LVBE\",\"item\":\"https:\\\/\\\/www.lvbe.uha.fr\\\/index.php\\\/equipe\\\/chercheurs-venus-collaborer-au-lvbe\\\/\"},{\"@type\":\"ListItem\",\"position\":4,\"name\":\"Dr. Peijie GONG\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/www.lvbe.uha.fr\\\/#website\",\"url\":\"https:\\\/\\\/www.lvbe.uha.fr\\\/\",\"name\":\"LVBE - Laboratoire Vigne, Biotechnologies, Environnement | Universit\u00e9 de Haute-Alsace\",\"description\":\"Campus Biop\u00f4le | Universit\u00e9 de Haute-Alsace\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/www.lvbe.uha.fr\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"fr-FR\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Dr. Peijie GONG - LVBE - Laboratoire Vigne, Biotechnologies, Environnement | Universit\u00e9 de Haute-Alsace","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.lvbe.uha.fr\/index.php\/equipe\/chercheurs-venus-collaborer-au-lvbe\/dr-peijie-gong\/","og_locale":"fr_FR","og_type":"article","og_title":"Dr. Peijie GONG - LVBE - Laboratoire Vigne, Biotechnologies, Environnement | Universit\u00e9 de Haute-Alsace","og_description":"Post Doctorant au LVBE 2018 Publications Zhang XT*, Gong PJ*(Co-first author), Shi YY, Wang YJ, Zhang CH, 2018. 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