{"id":3713,"date":"2022-04-14T10:41:29","date_gmt":"2022-04-14T08:41:29","guid":{"rendered":"https:\/\/www.lvbe.uha.fr\/en\/?page_id=3713"},"modified":"2022-04-14T10:41:29","modified_gmt":"2022-04-14T08:41:29","slug":"ph-d-gaelle-le-henanff","status":"publish","type":"page","link":"https:\/\/www.lvbe.uha.fr\/en\/team\/former-lvbe-ph-d-students\/ph-d-gaelle-le-henanff\/","title":{"rendered":"Ph. D. Ga\u00eblle LE HENANFF"},"content":{"rendered":"<h2><strong>Functional characterization of signaling genes involved in grapevine defence responses to biotic stresses<\/strong><strong>.<\/strong><\/h2>\n<p>Holded in September 2009<\/p>\n<p>Under the direction of Bernard WALTER.<\/p>\n<p>&nbsp;<\/p>\n<p><strong>Abstract<\/strong><\/p>\n<p><em>Vitis vinifera<\/em> is susceptible to many pathogens, thus requiring a massive use of phytochemicals. Alternative methods have to be developed. In the model plant <em>Arabidopsis thaliana<\/em>, the AtNPR1, AtNDR1 and AtEDS1 genes are components of the salicylic acid (SA) signalling pathway involved in resistance to biotrophic pathogens. Using a candidate gene approach, we have identified putative orthologs of these genes in the grapevine genome (VvNPR1.1, VvNPR1.2, VvNHL 1 and VvEDS1). Our work shows that VvNPR1-GFP fusion proteins are localized in the nucleus. Moreover, transient overexpression of VvNPR1 genes induces defence gene expression, in bath heterologous and homologous systems. Overexpression of VvNPR1.1 in npr1 mutants restores the different phenotypes of the mutants, while VvNPR1.2 overexpression does not. These results strongly suggest that VvNPR1.1 is the AtNPR1 ortholog. Overexpression of VvNHL 1 in ndr1 mutants does not restore resistance to <em>Pseudomonas syringae<\/em>. However, resistance to <em>Botrytis cinerea<\/em> in these plants is weakened, probably because of stimulation of cell death. Furthermore, VvNHL1 repression following <em>B. cinerea<\/em> infection in <em>V. vinifera<\/em> could be considered as a defence mechanism against necrotrophic pathogens and suggests that VvNHL1 is involved in promotion of cell death. VvEDS1 expression is induced by pathogens infection and SA treatment. VvEDS1 functional characterization is in progress using eds1 <em>Arabidopsis<\/em> mutants overexpressing VvEDS1. Knowledge of defence signaling pathways in <em>V. vinifera<\/em> will contribute to obtain pathogen resistant grapevines.<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Functional characterization of signaling genes involved in grapevine defence responses to biotic stresses. Holded in September 2009 Under the direction of Bernard WALTER. &nbsp; Abstract Vitis vinifera is susceptible to many pathogens, thus requiring a massive use of phytochemicals. Alternative methods have to be developed. In the model plant Arabidopsis thaliana, the AtNPR1, AtNDR1 and [&hellip;]<\/p>\n","protected":false},"author":5,"featured_media":0,"parent":44,"menu_order":2,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-3713","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.0 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Ph. D. Ga\u00eblle LE HENANFF - LVBE - Vine Biotechnology and Environment Laboratory<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.lvbe.uha.fr\/en\/team\/former-lvbe-ph-d-students\/ph-d-gaelle-le-henanff\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Ph. D. 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In the model plant Arabidopsis thaliana, the AtNPR1, AtNDR1 and [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.lvbe.uha.fr\/en\/team\/former-lvbe-ph-d-students\/ph-d-gaelle-le-henanff\/\" \/>\n<meta property=\"og:site_name\" content=\"LVBE - Vine Biotechnology and Environment Laboratory\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.lvbe.uha.fr\/en\/team\/former-lvbe-ph-d-students\/ph-d-gaelle-le-henanff\/\",\"url\":\"https:\/\/www.lvbe.uha.fr\/en\/team\/former-lvbe-ph-d-students\/ph-d-gaelle-le-henanff\/\",\"name\":\"Ph. D. 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