{"id":3696,"date":"2022-04-14T10:23:11","date_gmt":"2022-04-14T08:23:11","guid":{"rendered":"https:\/\/www.lvbe.uha.fr\/en\/?page_id=3696"},"modified":"2022-04-14T10:23:11","modified_gmt":"2022-04-14T08:23:11","slug":"ph-d-sylvain-lebel","status":"publish","type":"page","link":"https:\/\/www.lvbe.uha.fr\/en\/team\/former-lvbe-ph-d-students\/ph-d-sylvain-lebel\/","title":{"rendered":"Ph. D. Sylvain LEBEL"},"content":{"rendered":"<h2><strong>Characterization of Vitis vinifera PR10 genes and analysis of their expression during somatic embryogenesis<\/strong><\/h2>\n<p>Defended in December 2010<\/p>\n<p>Under the direction of Bernard WALTER and co-direction of Paul SCHELLENBAUM<\/p>\n<p><strong>Abstract<\/strong><\/p>\n<p>The objective of my work was to analyse the somatic embryogenesis process of <em>Vitis vinifera<\/em> at a molecular scale. Thus, the expression of genes implied in development or defence, especially PR10 genes, was monitored during the key-steps of entrance and exit of secondary somatic embryogenesis. The complete sequence of the <em>Vitis vinifera<\/em> genome available on the Genoscope website allowed the exhaustive characterization of the PR10 multigene family, which is constituted by 17 sequences localised on a tandem array on the chromosome 5. Among these 17 sequences, 3 are pseudogenes and at least 13 are transcribed sequences. The expression of 10 PR10 genes was first monitored in various grapevine tissues and in tissues after 2,4-D treatment using semi-quantitative RT-PCR. The results suggest a strong functional diversification. Moreover, the expression of several PR10 genes is high in embryogenic calli, suggesting that these genes could intervene in somatic embryogenesis. The expression of PR10 genes was also monitored in tissues showing different somatic embryogenic capabilities under 2,4-D treatment using quantitative RT-PCR. The results show that regulation of PR10 genes is dependent of the gene and tissue considered. Moreover, the expression of some genes is highly induced by 2,4-D treatment in tissues having embryogenic capability, while it is only weakly induced in tissues having no embryogenic capability, suggesting that these gene could be markers of embryogenic capability in grapevine.<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Characterization of Vitis vinifera PR10 genes and analysis of their expression during somatic embryogenesis Defended in December 2010 Under the direction of Bernard WALTER and co-direction of Paul SCHELLENBAUM Abstract The objective of my work was to analyse the somatic embryogenesis process of Vitis vinifera at a molecular scale. Thus, the expression of genes implied [&hellip;]<\/p>\n","protected":false},"author":5,"featured_media":0,"parent":44,"menu_order":6,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-3696","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.0 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Ph. D. Sylvain LEBEL - LVBE - Vine Biotechnology and Environment Laboratory<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.lvbe.uha.fr\/en\/team\/former-lvbe-ph-d-students\/ph-d-sylvain-lebel\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Ph. D. Sylvain LEBEL - LVBE - Vine Biotechnology and Environment Laboratory\" \/>\n<meta property=\"og:description\" content=\"Characterization of Vitis vinifera PR10 genes and analysis of their expression during somatic embryogenesis Defended in December 2010 Under the direction of Bernard WALTER and co-direction of Paul SCHELLENBAUM Abstract The objective of my work was to analyse the somatic embryogenesis process of Vitis vinifera at a molecular scale. Thus, the expression of genes implied [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.lvbe.uha.fr\/en\/team\/former-lvbe-ph-d-students\/ph-d-sylvain-lebel\/\" \/>\n<meta property=\"og:site_name\" content=\"LVBE - Vine Biotechnology and Environment Laboratory\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.lvbe.uha.fr\/en\/team\/former-lvbe-ph-d-students\/ph-d-sylvain-lebel\/\",\"url\":\"https:\/\/www.lvbe.uha.fr\/en\/team\/former-lvbe-ph-d-students\/ph-d-sylvain-lebel\/\",\"name\":\"Ph. D. 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