Dr. Peijie GONG

Post Doctorant

Courriel : peijie.gong@uha.fr

Education

Oct.2013-Jun.2017

Doctor’s degree in Biology

Botanical Institute, Karlsruhe Institute of Technology (KIT), Germany
Major: Molecular and cell biology
Thesis title: Die to Survive, Functional Analysis of Grapevine Metacaspases responsive to ETI (effector-triggered immunity) -related cell death
(Supervisor: Prof. Dr. Peter Nick)
Overall mark: very good (magna cum laude)

Sep.2010-Jun.2013

Master’s degree in Agronomy

College of Horticulture, Northwest A&F University
Major: Pomology
Thesis title: Characterization of Caspase-like Gene Family Related to Ovule Abortion in Grape (Vitis vinifera)
(Supervisor: Prof. Zhang Chaohong, Prof. Wang Yuejin)
GPA: 88.3/100

Sep.2006-Jul.2010

Bachelor’s degree in Agronomy

College of Agronomy, Gansu Agricultural University
Major: Horticulture
Thesis title: The Identification of Genetic Relationship in Purple Globe Grape Using RAPD Molecular Marker
GPA: 80.28/100

Scientific skills / techniques

  • Samples collection, isolation of genomic DNA and RNA
  • Pathogen cultivation and inoculation
  • Suspension cell cultivation and treatment
  • TA gene cloning, 5’RACE, 3’RACE, sequence analysis
  • RT-PCR, quantitative PCR
  • GATEWAY techniques, related vector construction
  • Crisp-cas knock out technique
  • Transient transformation via biolistic bombantment
  • Stable transformation using Agrobacterium-mediated transformation
  • Operation of fluorescence confocal microscopy (Spining Disc, Aptom, Zeiss)
  • Promoter localization and cloning
  • Cis-elements in promoter sequences analysis (PLANTCARE, PLACE)
  • Promoter activity assay via dual luciferase reporter system
  • Protein extraction and SDS-PAGE analysis
  • preparation of tag-fusion protein and polyclonal antibodies
  • West-blot analysis

Research Experience

Dec.2017 - Present

Postdoc position, in Université de Haute-Alsace (UHA)(supported by Vitifutur project of EU

Analysis and engineering of programmed cell death in grapevine trunk disease (GTD)
Responsibilities:

  • Resolving core question: How to use caspase-like enzyme to rescue our grape host from GTD pathogens infection?
Oct. 2013 – Sep. 2017

Doctor’s degree dissertation, (supported by Chinese Scholarship Council)

Function analysis of metacaspase family genes and their promoters during ETI-triggered PCD in Vitis defense system
Responsibilities:

  • Setting up expression pattern of each metacaspase family member during HR process in grapevine
  • HR-like necrotic spots observed on Rpv3+ grapevine induced by viticola 1191-B15,1137-C20
  • Clarifying cellular location and cellular function of VrMC2 and VrMC5 in PCD process in rupestris
  • Supervising the thesis of bachelor students, master students
  • Giving exercises and lectures to bachelor students
Sep. 2011 – Jun. 2013

Part for Master’s degree thesis, (supported by China Agriculture Research System (30)

Characterization of metacaspase gene family of vitis vinifera and their differential expression analysis during ovule abortion process of stenospermocarpic seedlessness
Responsibilities:

  • Identification and isolation of family genes
  • Chromosome localization of each gene
  • Multiple-sequence alignments and phylogenetic analysis
  • Cis-elements in promoter sequences analysis
  • Gene expression of metacaspase analyzed by QRT-PCR in different stages of ovule development
Sep. 2011– Jun. 2013

Part of Master’s degree thesis, (supported by Natural Science Foundation of China (Grant no. 31171926)

VPE, a novel vacuolar processing enzyme gene family from Vitis vinifera, functions analysis in ovule development process of grapevine
Responsibilities:

  • The analysis of βVPE gene polymorphism from grapes and the its function with the seedlessness character (in Arabidopsis)
  • Cloning and analysis of VPE family and the differentially expressed gene in ovule abortion of seedless grape
  • Function deletion analysis of β VPE promoter from seedless grapes (in Arabidopsis)

Languages and Software skills

Chinese: Mother tongue
English: Fluent
German Basic: B1

Proficient in Windows and Office software (Word, Excel, Powerpoint, Origin, PS, etc.)

Proficient in biology related software: MEGA, Lasergene, Serial Cloner, Primer Premier 5, Bio-Rad CFX manager, Zeiss Microscopy, Leica Microscopy, DNAMAN, and ENDNOTE etc.

Publications

  • Zhang XT*, Gong PJ*(Co-first author), Shi YY, Wang YJ, Zhang CH, 2018. Genetic
    Inter-relationships among Chinese Wild Grapes based on SRAP Marker analyses,
    Vitis, 57, 151–
    157.
  • Peijie Gong, Yan Li, Yujin Tang, Rong Wei, Zhu Huijun, Yuejin Wang, Chaohong Zhan. Vacuolar processing enzyme (VvβVPE) from Vitis vinifera, processes seed proteins during ovule development, and accelerates seed germination in VvβVPE heterologously over-expressed Arabidopsis. Plant Science, Volume 274, 2018, Pages 420-431, ISSN 0168-9452. https://doi.org/10.1016/j.plantsci.2018.06.023.
  • Gong PJ, Riemann M and Nick P 2017. Expression and function analysis of metacaspase gene family during pathogen-triggered PCD in grapevine (being composing)
  • Zhang CH*, Gong PJ* (Co-first author), Wei R, Li SX, Zhang XT, Yu YH, Wang YJ. 2013. The metacaspase gene family of Vitis vinifera L. : Characterization and differential expression during ovule abortion in stenospermocarpic seedless grapes. Gene 528, 267-276.
  • Tang YJ, Wang RP, Gong PJ, Li SX, Wang YJ, Zhang CH. 2016. Gene Cloning, Expression and Enzyme Activity of Vitis vinifera Vacuolar Processing Enzymes (VvVPEs). Plos One 11.
  • Ji W, Li ZQ, Yao WK, Gong PJ, Wang YJ. 2013. Abnormal Seedlings Emerged during Embryo Rescue and Its Remedy for Seedless Grape Breeding. Korean Journal of Horticultural Science & Technology 31, 483-489.
  • Gong PJ, Zhang CH, Wei R, Li SX, Wang YJ. 2013. Fusion Expression and Purification of β Type Vacuole Processing Enzyme Gene (VvβVPE) in Vitis vinifera and Its Antibody Preparation. Journal of Agricultural Biotechnology, 21(6): 659-667. (In Chinese)
  • Li SX, Zhang CH, Gong PJ, Yang K, Zhang XT, Wang YJ, 2012. Cloning and Expression Analysis of the Differentially Expressed VvWRKY20 Gene in the Development of Seedlessness Grape (Vitis vinifera) Ovule Abortion, Journal of Agricultural Biotechnology, 20(10): 1143-1149 (In Chinese)
  • Wei R,Gong PJ,Li SX,Wang YJ,Zhang CH, 2014. Cloning and Expression Analysis of TIAR-like gene in the development of grape (Vitis vinifera) ovule. Journal of Fruit Science, 31(4) : 551-557 (In Chinese)